FIG. 3.

(A) Structures of the promoter-reporter integration vectors pLB85 and pLB86. The direction of transcription is indicated by arrows. The black box indicates the 207-bp attP region of TP901-1. (B) Results of site-specific integration of the promoter-reporter vectors in the chromosome of L. lactis subsp. cremoris. The multiple cloning sites (mcs) are indicated by horizontal lines, and the direction of transcription is indicated by arrows. The black box indicates the attP part of attL and attR of TP901-1, and the thin black arrow indicates the orientation of transcription of chromosomal genes located within the attB region (8).