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. 2022 May 11;34:11–21. doi: 10.1016/j.jot.2022.04.002

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Effects of MFG-E8 on macrophage polarisation (A) The morphology of BMDMs were observed using a microscope after IFN-γ+ LPS, IL-4 or MFG-E8 stimulation for 24 h (B) The activation-related surface markers of BMDMs were analysed by FCM. When compared with the control group (untreated), a significant shift of CD86+ and CD206+ macrophages were observed in the IFN-γ+ LPS and IL-4 groups, respectively, at 24 h after stimulation, but not in the MFG-E8 group. N = 3 per group (C) Relative mRNA expression levels of inflammatory cytokines were detected in BMDMs after different stimulations for 24 h N = 3 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 (D) IF staining of iNOS or CD206 demonstrated that MFG-E8 could partially reverse the expression of iNOS in M1 macrophages, but could not increase the CD206 expression in M2 macrophages. Scale bar = 100 μm N = 4 per group. ∗∗P < 0.01. BMDM, bone marrow derived macrophage; IFN-γ, interferon γ; LPS, lipopolysaccharide; IL-4, interleukin-4; MFG-E8, milk fat globulin protein E8; FCM, flow cytometry.