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. 2022 May 11;34:11–21. doi: 10.1016/j.jot.2022.04.002

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Efferocytosis assay (A) Different doses of MFG-E8 were co-cultured for 2 h, and the clearance of apoptotic PMNs by BMDMs was viewed by fluorescent staining. Apoptotic PMNs appeared in green and BMDMs appeared in red. Scale bar = 100 μm (B) The relative efferocytosis index were calculated from fluorescent staining images. Increasing concentrations of MFG-E8 generated a parabolic-shaped response curve for the efficiency of efferocytosis. N = 3 per group. ∗∗P < 0.01, ∗∗∗P < 0.001 (C) The expression levels of iNOS and CD206 were evaluated by western blot analysis. N = 3 per group. ∗∗P < 0.01, ∗∗∗P < 0.001. MFG-E8, milk fat globulin protein E8; BMDM, bone marrow derived macrophage; PMN, polymorphonuclear neutrophil.