Fig. 4.

ApoEV metabolism shows a wave-like movement pattern. (A) Immunofluorescent staining shows the proliferate rate change in the skin on days 1, 3 and 7 with or without apoEVs injection, indicated by EdU staining. The activity of Wnt/β-catenin pathway and the elimination of apoEVs are observed at the same time. White dotted line represents the dividing layer between stratum corneum and the other layers of epidermis. (B) Quantification graph shows the change of EdU positive cell ratio and fluorescent intensity of Wnt/β-catenin in the skin from day 1 to day 7 after apoEVs injection. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA test. Data shown as mean ± SD. (C) Immunofluorescent staining shows the wave pattern when apoEVs are cleared in the skin, but XAV939 treatment interferes with the pattern, as indicated by the schematic graph. The white dotted lines simulate the pattern of apoEVs. SC, stratum corneum; BM, basement membrane; D, dermis. Scale bar (A), 50 μm; Scale bars (C), 5 μm.