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Activity of 34b in HEK293 cellular assays. (a) Activity of 34b in an ERK5:MEF2D luciferase reporter assay examining ERK5 ΔTAD, a truncated form of ERK5 containing the kinase domain but lacking the C-terminal extension, or full-length ERK5 (mean ± SEM, n = 5 separate experiments); (b) growth inhibition following a 72 h incubation with compound 34b (mean ± SEM, n = 8 separate experiments).
