Figure 4.

Continuous inhibition of HDACs 1 and 3 by compounds 4 and 5. (A) (top) Continuous assay progression curves and (bottom) k_obs secondary plots (mechanism B) for compound 4. (B) (top) Continuous assay progression curves and (bottom) k_obs secondary plots (HDAC1 data fitted to mechanism A and HDAC3 data fitted to mechanism B) for compound 5. Data represent mean ± SEM of individual experiments, with each experiment performed at least twice. See Figure S6 for HDAC2 data and Table 1 for numerical data. *The HDAC3 preparation contained the DAD of NCoR2.