Table 1.
Filter set specifications
| Filter set | Excitation [nm] | Emission [nm] | Exposure time [ms] | Tested dyes |
|---|---|---|---|---|
| FS395 | 364–366 | 381–403 | 1,000 | Hoechst 33342, DAPI, BUV395 |
| FS421 | 370–410 | 440–485 | 50 | BV421, eF450, PB, Hoechst, DAPI |
| FS488 | 450–490 | 500–550 | 500 | FITC, AF488, GFP, Opal520 |
| FS560 | 525–575 | 570–640 | 300 | PE, eF570, Opal570 |
| FSPerCP | 456–484 | 672–748 | 300 | PerCP, PerCP-Cy5.5, Opal650 |
| ALL (Bleach) | 390–644 | – | 20,000 | – |
BV = Brilliant Violet, BUV = Brilliant Ultraviolet, eF = eFluor, PB = Pacific Blue, DAPI = 4′,6-Diamidino-2-phenylindole, FITC = Fluorescein isothiocyanate, AF = Alexa Fluor, GFP = Green Fluorescent Protein, PE = Phycoerythrin, PerCP = Peridinin-Chlorophyll-Protein.