Fig. 6.

IL-4 and IL-13 receptor subunit expression on monocytes and MDMs, and induction of SOCS1 in AMs of healthy and asthmatic men and women. PBMCs were isolated from the peripheral blood of healthy and allergic asthmatic study participants, stained as in Fig. 2 for monocyte subsets and for IL-4 and IL-13 receptor subunits, and analyzed by flow cytometry. Monocytes were gated on live cells, CD45⁺CD3⁻CD19⁻CD20⁻CD56⁻CD66b⁻, and either CD14⁺ or CD16⁺. Analysis of (A) gamma C (γC), (B) IL-4Rα, (C) IL-13Rα1, and (D) IL-13Rα2 in “classical” and “non-classical” monocytes. MDMs were identified as live CD45⁺CD14⁺CD16⁺ cells. Analysis of (E) γC, (F) IL-4Rα, (G) IL-13Rα1, and (H) IL-13Rα2 in MDMs. The change in mean fluorescence intensity (MFI) of the different IL-4 and IL-13 receptor subunits (MFI target – MFI isotype) is shown. The number of donors in each group is the same as in Fig. 1. (I) AMs were isolated from the lungs of healthy (n = 6) and asthmatic (n = 4) subjects and stimulated with IL-4 (20 ng/mL) for 0, 2, 6, or 24 h. Expression of SOCS1 was determined by qPCR using the ΔΔ cycle threshold (CT) method. Amounts of mRNA were compared to those in the unstimulated sample of each donor. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.