Skip to main content
. Author manuscript; available in PMC: 2022 May 16.
Published in final edited form as: Cell Rep. 2022 Apr 19;39(3):110711. doi: 10.1016/j.celrep.2022.110711

Figure 2. Lmx1b and Pet1 act in adult 5-HT neurons to ensure survival of pericellular baskets.

Figure 2.

(A) Coronal schematic highlighting location (red box) of pericellular baskets in the dorsolateral septum (dLS).

(B–D) Left, representative confocal images (63×) of TdTomato+ (anti-RFP) light pericellular baskets (B), dense baskets (C), and dense basket remnants (D) at 1 year post-TAM. Right, quantification of basket or remnant numbers relative iCON numbers; ±SEM; n = 3 mice per genotype; 2–3 sections per animal were used for counting; two-way ANOVA with Welch’s correction; unpaired t test with Welch’s correction for iCON versus iPKO. Scale bars, 10 μm.

(E) Top, immunohistochemical analysis of synaptic triads in dLS at 6 months post-TAM, identified with RFP, presynaptic marker, synapsin 1 (Syn1), postsynaptic marker gephyrin (Gphn), and DAPI. Bottom, highlights overlap of pre- and post-synaptic makers. Right, relative numbers of triads; ±SEM; n = 3 mice with three sections analyzed per animal; one-way ANOVA. Scale bar, 5 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant. See also Figure S2.