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. 2022 May 16;13:206. doi: 10.1186/s13287-022-02884-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Trilineage differentiation of unlabeled (UL-MSCs) and 6h-MIRB-labeled (6-h MIRB-MSCs) integrin α10-MSCs. A Chondrogenic differentiation was induced and evaluated by qPCR analysis of collagen type II (COL2), aggrecan and integrin α10 in UL-MSCs or 6h-MIRB-MSCs. Uninduced UL-MSCs and 6h-MIRB-MSCs served as controls. The relative quantity of all genes was normalized to chondrogenically induced UL-MSCs. Uninduced integrin α10-MSCs (both UL-MSCs and 6h-MIRB-MSCs) showed no expression of COL2 or aggrecan but did show expression of integrin α10. IntgA10 = integrin α10; MIRB = Molday Ion conjugated with Rhodamine B. B Adipogenic and osteogenic differentiation was confirmed by positive staining of intracellular lipid droplets with Oil Red O and extracellular calcium deposits with Alizarin Red, respectively. There was no visual difference between UL-MSCs or 6h-MIRB-MSCs