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. 2022 May 16;27:39. doi: 10.1186/s11658-022-00331-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — Knockdown of LINC00470 suppresses ESCC cell proliferation, G1/S transition, and migration in vitro. A Transfection with si-LINC00470 dramatically suppressed LINC00470 expression in EC9706 and TE-9 cells. B CCK-8 assay showed that knockdown of LINC00470 resulted in growth retardation of EC9706 and TE-9 cells. Flow cytometry assay was conducted to analyze cell cycle distribution in transfected EC9706 C and TE-9 D cells. E Cell migration was evaluated in transfected EC9706 and TE-9 cells by transwell assay. Magnification, ×200; scale bar, 100 μm. F Western blot analysis was performed to determine the protein levels of PCNA, CDK4, E-cadherin, and N-cadherin in EC9706 and TE-9 cells. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001, compared with si-NC