Fig. 2.

Concentration of vitellogenin (VTG) over time in plasma of male fathead minnows exposed to A,B Control (0.00 ng/L, black circle), A High (5.48 ng/L, orange triangle), or B Low (1.33 ng/L, blue square) EE2 for 7 days followed by a 14-day depuration in clean water. Each point represents the mean with vertical lines displaying standard deviation (n = 3–5). Vertical dashed gray line indicates the beginning of the depuration period. Horizontal dotted line marks VTG (ng mL⁻¹) = 0. Asterisks indicate significant difference between the means of Control and Low or High within that day, determined by Welch’s ANOVA with Games-Howell post-hoc test (*p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001). Individual sample values may be found in Supplementary Fig. S5.