Fig. 3.

Expression of vitellogenin (vtg) over time in liver of male fathead minnows exposed to A,B Control (0.00 ng/L, black circle), A High (5.48 ng/L, orange triangle), or B Low (1.33 ng/L, blue square) EE2 for 7 days followed by a 14-day depuration in clean water. Each point represents the mean with vertical lines displaying standard deviation (n = 8 – 10, Day 21 n = 4). Vertical dashed gray line indicates the beginning of the depuration period. Asterisks indicate significant difference between the means of Control and Low or High within that day, determined by Welch’s ANOVA with Games-Howell post-hoc test (*p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001). Individual sample values may be found in Supplementary Fig. S6.