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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Ecotoxicol Environ Saf. 2022 Mar 30;236:113428. doi: 10.1016/j.ecoenv.2022.113428

Table 2.

Costs are rounded to the nearest dollar based on current list price.Sample prep = TECO Mucus collection set vs. TRIzol + Phasemaker tubes + TURBO DNA-free Per sample assay cost = 2 replicates of one dilution in TECO Cyprinid Vitellogenin ELISA vs. 3 replicates of 4 target genes in RNA-to-Ct™ 1-Step Kit.

ELISA RT-qPCR
Total cost per sample $31 $14
Sample collection/prep $5 $5
Per sample assay cost $26 $9
Intraplate variability 16% ≤ 3%
Interplate variability 14% ≤ 3%
Time (sample collection to data acquisition) 1 day 2 days