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. 2022 May 13;42(5):BSR20212418. doi: 10.1042/BSR20212418

Figure 5. IL-6 specifically induces LCN2 promoter activity under control of the NF-κB and STAT3 signaling pathways.

Figure 5

(A) Human CRC cells were transfected with reporter plasmid carrying a 1233-bp fragment of the LCN2 promoter, then the growth medium supplemented with IL-6 was added to the cells. After 24 h, the cells were harvested and promoter activities were determined. The data represent the mean ± SE of three independent experiments. **P<0.01 versus unstimulated cells. (B) After transfection with LCN2 promoter construct, IL-6 and specific inhibitors were added to DLD-1 cells for 24 h. Then, the promoter activities of LCN2 were determined (left). DLD-1 cells were co-transfected with LCN2 promoter construct and indicated siRNA, then the cells were stimulated with IL-6 for 24 h. Alterations in LCN2 promoter activities were detected. The data represent the mean ± SE of three independent experiments **P<0.01 versus unstimulated cells or stimulated cells.