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Oligonucleotide probe sequences and hybridization temperatures used for the probe analysis of clone libraries obtained from particle-associated and planktonic samples from the northwestern Mediterranean Seaa
| Probe | Sequence (5′-3′) | Target group | Temp (°C) |
|---|---|---|---|
| β-AO233 | AGCTAATCAGRCATCGG | All β-subgroup ammonia oxidizers | 44 |
| Nsp436 | TTTCGTTCCGGCTGAAAG | All Nitrosospira spp. | 45 |
| Nmo254 | GTAGGCCSTTACCCYACC | All Nitrosomonas spp. | 43 |
The probes were designed and optimized by Stephen et al. (40).
