TABLE 3.
Percentages of clones in clone libraries derived from particle-associated and planktonic material from the northwestern Mediterranean Sea that hybridized with probes β-AO233, Nsp436, and Nmo254
| Clone library | No. of clones | Clones that hybridized witha:
|
|||||
|---|---|---|---|---|---|---|---|
| β-AO233
|
Nsp436
|
Nmo254
|
|||||
| No. | % | No. | % | No. | % | ||
| 100 FREE | 90 | 0 | 0 | 0 | 0 | 0 | 0 |
| 100 AGG | 90 | 8 | 9 | 0 | 0 | 8 | 100 |
| 400 FREE | 89 | 23 | 26 | 22 | 96 | 1 | 4 |
| 400 AGG | 90 | 15 | 17 | 0 | 0 | 15 | 100 |
| 700 FREE | 91 | 6 | 7 | 1 | 17 | 5 | 83 |
| 700 AGG | 87 | 72 | 83 | 3 | 4 | 69 | 96 |
a
The results were corrected for clones which gave unexpected probe profiles and which after sequence analysis proved to be closely related to non-ammonia-oxidizing species. The values for Nsp436 and Nmo254 were calculated as percentages of the number of β-AO233-positive clones.