FIG. 1.
Construction of different expression vectors derived from plasmids pHES12 and pCYTEXP1. The inserts and restriction sites used for cloning are given. (A) P. cepacia expression vector (pHES12) containing the complete lipase operon (lipase and chaperone) under the control of the tac promoter. (B) E. coli expression vector (pCYTEXP1) containing the strong, temperature-inducible λPRL promoter. ORI, origin. (C) Inserts of pCYTEXP1-derived expression vectors. N, NdeI; E, EcoRI. Numbers in parentheses are base pairs. See Table 1, footnote a, for explanations of designations.