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SDS-PAGE of overexpressed and Ni-NTA-purified lipase and chaperone in E. coli. Proteins were stained with Coomassie brilliant blue R-250. Lanes: 1, E. coli pT-Lip-Hp cell lysate; 2, lipase inclusion bodies isolated from E. coli pT-Lip-Hp cell lysate; 3, E. coli pT-Δ70HpHis cell lysate; 4, Ni-NTA-purified Δ70HpHis; 5, E. coli pT-ompAΔ70HpHis cell lysate; 6, Ni-NTA-purified ompAΔ70HpHis; 7, E. coli pT-Δ34HpHis cell lysate; 8, E. coli pT-ompAΔ34HpHis cell lysate.
