Table 2.

GDA Reaction Rates

	HPLC						absorbance			emission
	λ mntr a	k 1 b	t 1/2 c	K M d	k 2 d	k2/KMd	λ mntr a	k 1 b	t 1/2 c	λ mntr a	k 1 b	t 1/2 c
G to X	260	26 ± 2	27	12 ± 9	36 ± 27	3.0 ± 0.3	270	22 ± 1	31
thGN to thXN	320	NR	NR	NR	NR	NR	355	NR	NR	450e	NR	NR
tzGN to tzXN	260	32 ± 1	21	8 ± 3	32 ± 7	4.2 ± 1.0	355	25 ± 1	27	450f	24 ± 1	29

^aλ_mntr reported in nm and represents wavelength monitored.

^bPseudo-first-order reaction kinetics slope of the exponential approximation in 10⁻³ s⁻¹.

^ct_1/2 is reaction half-life calculated assuming pseudo-first-order kinetics.

^dK_M, k₂, and k₂/K_M are reported in μM, s⁻¹, and 10⁶ M⁻¹ s⁻¹, respectively.

^eλ_exc at 360 nm for ^thG_N reactions.

^fλ_exc at 328 nm for ^tzG_N reactions. Experiments done in triplicate.