Figure 1.

Mtb induces Irg1 protein expression in macrophages. BMDMs obtained from transgenic mice expressing Irg1-GFP protein (Irg1-GFP⁺) were infected with H37Rv-RFP at different MOI (1:1; 5:1; 10:1) as indicated. (A, B) Mtb-induced Irg1-GFP expression was evaluated by flow cytometry at 6 h and 24 h p.i. (A) Sample FACS plots and (B) frequency of macrophages expressing Irg1-GFP are shown. (C) Histogram FACS plot and geometric MFI of Irg1-GFP was evaluated by flow cytometry at 6 h (left) and 24 h (right) p.i. (D) FACS plot and histogram of Irg1-GFP expression in cells displaying low, intermediate and high MFI of RFP. Statistical significance was assessed by one-way ANOVA analysis for the indicated experimental condition (***p < 0.001). The data shown are from a representative experiment of two performed.