Skip to main content
. 2022 May 2;12:862582. doi: 10.3389/fcimb.2022.862582

Figure 2.

Figure 2

Mtb induced Irg1 expression is partially dependent on TLR-2/MyD88/NFκB signaling. (A, B) Irg1 mRNA expression was assessed in C57BL/6, TLR-2-/-, MyD88-/- and TLR-4-/- and NFkB-p50-/- BMDM cultures following H37Rv infection at 6 h and 12 h p.i. (C) Irg1mRNA levels were evaluated in C57BL/6 BMDMs at 6 h following Pam3CSK4 (10 ng/ml) or LPS (10 ng/ml) stimulation. (D, E) Irg1 mRNA expression was assessed in BMDMs from (D) C57BL/6, TLR-9-/- and (E) CARD9-/- mice infected with H37Rv strain (MOI of 1) at 6 h p.i. C57BL/6 BMDMs were stimulated with (D) CpG (2 µM) or (E) trehalose dimycolate (50 µg/well) and Irg1 expression evaluated at 6 h after stimulation. As a control both CpG and trehalose dimycolate were shown to induce significant TNF-α responses when tested under the same conditions (data not shown). Significant differences are indicated as follows: *p<0.05, and ***p < 0.001. Results are representative of at least two separate experiments performed.