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. 2022 May 2;12:862582. doi: 10.3389/fcimb.2022.862582

Figure 5.

Figure 5

Mtb-induced Type I interferon signaling plays a major role in Irg1 expression. (A) mRNA levels of Irg1, Tnf, Ifnb1, Oas1a and Mx2 were measured in IFNAR-/- and C57BL/6 BMDMs at 6 h following H37Rv Mtb infection or LPS stimulation (10 ng/ml). (B) Irg1 mRNA expression was assessed in C57BL/6 BMDMs treated or not with recombinant IFN-β (10 ng/ml). (C) mRNA levels of Irg1 were evaluated in C57BL/6 BMDMs pretreated with actinomycin D (ActD) (1 µg/ml) or cycloheximide (CHX) (10 µg/ml) for 2 h and then infected with H37Rv Mtb for 6 h. (D) Irg1 mRNA expression was assessed in Mtb-infected IFNγR-/- and C57BL/6 macrophage cultures in the presence of recombinant IFNγ (10U/ml) and/or IFNβ (10 ng/ml) at 6 h p.i. Significant differences are indicated with asterisks (***p < 0.001). The data shown are representative of two separate experiments performed.