Table 4.
P-values for fold-changes in nCounter PCR for difference between B6[WT-MUT] AND NSG[WT-MUT] groups. Concordance of results to RNA sequencing values exists for all gene except Casp8 and Pycard, the former showing equal upregulation of both groups, the latter showing no change in expression between the two groups. No significant difference was noted between any of Il18, Il1 or Il6 values.
| Gene target | p-value B6[WT-MUT] versus NSG[WT-MUT] |
|---|---|
| Aim2 | 0.8752 |
| Casp1 | 0.1238 |
| Casp8 | 0.9022 |
| Ctsb | 0.5808 |
| Naip | 0.0159 |
| Nfkb2 | 0.1364 |
| Nlrc4 | 0.0483 |
| Nlrp1 | 0.0983 |
| Nlrp3 | 0.0583 |
| P2rx7 | 0.0750 |
| Pycard | 0.7832 |
| Il18 | 0.1558 |
| Il1b | 0.2096 |
| Il6 | 0.7497 |
| Gapdh | 0.8720 |
| Hprt | 0.0585 |
| Pgk1 | 0.0365 |