Table 2. Annotation of 20 viruses associated with nudibranch samples.
| Contig ID | Size (bp) | NCBI nt database (DIAMOND) | Phylogenetic classification (CAT) | Reported host | Suggested host (palmID) |
|||
|---|---|---|---|---|---|---|---|---|
| Description | Phylum | Order | Family | Species | ||||
| SRR3726693_23 | 6,219 | Riboviria sp. isolate 3nj-RDRP-3 | Beihai picorna-like virus 75 | Echinoderm | Metagenome | |||
| SRR1950953_7108 | 9,998 | Riboviria sp. isolate 3nj-RDRP-3 | Beihai picorna-like virus 75 | Echinoderm | Metagenome | |||
| SRR1950940_818 | 8,834 | Beihai picorna-like virus 72 | Pisuviricota | Beihai picorna-like virus 72 | Anthozoa | Bacteria (Fig. 3A) | ||
| SRR1950953_2265 | 13,015 | Beihai charybdis crab virus 1 | Crustacea | Gastropod (Fig. 3B) | ||||
| SRR1950953_444 | 10,163 | Beihai picorna-like virus 75 | Beihai picorna-like virus 75 | Echinoderm | Metagenome | |||
| SRR3726693_131 | 3,162 | Riboviria sp. isolate 3nj-RDRP-3 | Beihai picorna-like virus 75 | Echinoderm | Metagenome | |||
| SRR1950953_981 | 11,238 | Wenzhou picorna-like virus 46 | Pomacea canaliculata | Metagenome (viral - porifera) | ||||
| SRR1950949_2406 | 9,654 | Bacteria (Fig. 3C) | ||||||
| SRR3726693_286 | 10,990 | Wuhan snail virus 2 | Mollusca | |||||
| SRR3726693_3931 | 9,200 | Wenzhou picorna-like virus 46 | Pomacea canaliculata | Metagenome (viral - porifera) | ||||
| SRR3726693_6245 | 6,102 | Beihai picorna-like virus 56 | Pisuviricota | Beihai picorna-like virus 56 | n.d. | |||
| SRR1950942_4074 | 8,133 | Wenzhou picorna-like virus 19 | Pisuviricota | Wenzhou picorna-like virus 19 | Gastropoda | Plant (Fig. 3D) | ||
| SRR1950951_5889 | 6,244 | Basavirus sp. isolate BaV/21164 | Bacteria (Fig. 3E) | |||||
| SRR3726705_1238 | 3,321 | Bacteria (Fig. 3F) | ||||||
| SRR3726699_38607 | 4,326 | Eptesicus fuscus rhabdovirus | ||||||
| SRR1950948_28126 | 3,196 | Barns Ness breadcrumb sponge hepe-like 1 | Kitrinoviricota | Hepelivirales | Hepeviridae | Barns Ness hepe-like virus 1 | Porifera | Arthropoda (Fig. 3H) |
| SRR1950948_38692 | 1,519 | Barns Ness breadcrumb sponge hepe-like 2 | Kitrinoviricota | Hepelivirales | Hepeviridae | Barns Ness hepe-like virus 2 | Porifera | |
| SRR3726694_24261 | 3,010 | Negarnaviricota | Mononegavirales | Nyamiviridae | ||||
| SRR3726696_22111 | 4,656 | Biomphalaria virus 4 | Pisuviricota | Picornavirales | Biomphalaria virus 4 | Biomphalaria | Bacteria (Fig. 3H) | |
| SRR3726698_7138 | 1,701 | Hubei earwig virus 1 | Dermaptera | |||||
Note:
For each contig, identified by the contig ID and size, the annotation obtained using blastn against the NCBI nt database (description) and the taxonomic classification obtained using CAT (phylum, order, family and species) were reported together with the suggested host of the best hit or by palmID. The viral contigs were ordered by total number of mapped reads. Underlined hits referred to genomes including low-confidence RdRPs (no palmID analysis was performed). Bolded taxonomic classifications referred to information not retrieved by CAT but added by palmID. SRR3726698_7138 represented the hit identified by palmscan and Virsorter2 only (annotations was retrieved using blastx).