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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Nat Protoc. 2021 Jun 28;16(8):3874–3900. doi: 10.1038/s41596-021-00562-w

Table 2.

Base medium composition

Component Stock concentration Volume added Final concentration Recommended storage condition
Advanced DMEM/F12 NA 500 ml NA 4 °C fridge
HEPES buffer 1 M 5 ml 10 mM 4 °C fridge
Glutamax 200 mM 5 ml 2 mM 4 °C fridge
Penicillin/Streptomycin* 10,000 units/ml 5 ml 100 units/ml −20 °C freezer
Final volume 515 ml
*

This component is excluded for antibiotic-free culture medium. NA: not applicable. HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (CAS no. 7365-45-9). DMEM/F12: Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium.