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. 2022 Apr 5;322(6):G598–G614. doi: 10.1152/ajpgi.00017.2022

Figure 3.

Figure 3.

Morphological and molecular characterization of human Barrett’s organoids derived from endoscopic biopsy specimens. Representative images of BAR-B24 organoids (derived from biopsy tissue specimens) at day 14. A: H&E and Alcian blue staining demonstrating a single layer of columnar cells with some goblet-type cells (arrows). Scale bar = 50 µm; biological replicates = 3. DIC and immunofluorescence images for columnar markers MUC2, MUC5AC, and CKs 20, 8/18, and 7 (B), squamous basal cell markers CK14 (red in NES-D5 organoids, green in BAR-B24 organoids) and p63 (C) and esophageal submucosal gland putative markers LEFTY1 and OLMF4 in BAR-B24 organoids (D); NES-D5 esophageal squamous cell organoids derived from the normal esophageal epithelium of an organ donor served as a control. DAPI was used as a nuclear counterstain. Scale bar = 50 µm. DAPI, 4′,6′-diamidino-2-phenylindole; DIC, differential interference contrast microscopy; H&E, hematoxylin and eosin; MUC2, mucin 2.