Table 1. Primers and amplicon sizes of the amplified products and specific annealing temperatures.

M.Os	Target gene	Primers sequences	Amplicon size (bp)	Amplification (35 cycles)
				Denaturation	Annealing	Extension
E. coli	fimH	TGCAGAACGGATAAGCCGTGG GCAGTCACCTGCCCTCCGGTA	508	94°C 30 seconds	50°C 45 seconds	72°C 45 seconds
	papC	TGATATCACGCACTCAGTAGC CCGGCCATATTCACATAA	501	94°C 30 seconds	58°C 40 seconds	72°C 45 seconds
	iss	ATGTTATTTTCTGCCGCTCTG CTATTGTGAGCAATATACCC	226	94°C 30 seconds	54°C 30 seconds	72°C 30 seconds
	iutA	GGCTGGACATGGGAACTGG CGTCGGGAACGGGTAGAATCG	300	94°C 30 seconds	63°C 30 seconds	72°C 30 seconds
	blaCTX-M	ATG TGC AGY ACC AGT AAR GTK ATG GC TGG GTR AAR TAR GTS ACC AGA AYC AGC GG	593	94°C 30 seconds	54°C 40 seconds	72°C 45 seconds
	bla TEM	ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC	516	94°C 30 seconds	54°C 40 seconds	72°C 45 seconds
	tetA	GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA	576	94°C 30 seconds	50°C 40 seconds	72°C 45 seconds
S. aureus	mecA	GTA GAA ATG ACT GAA CGT CCG ATA A CCA ATT CCA CAT TGT TTC GGT CTA A	310	94°C 30 seconds	50°C 30 seconds	72°C 40 seconds
	coa	ATA GAG ATG CTG GTA CAG G GCT TCC GAT TGT TCG ATG C	630	94°C 30 seconds	55°C 40 seconds	72°C 40 seconds
	spa	TCA ACA AAG AAC AAC AAA ATG C GCT TTC GGT GCT TGA GAT TC	226	94°C 30 seconds	50°C 30 seconds	72°C 40 seconds
	tetK	GTAGCGACAATAGGTAATAGT GTAGTGACAATAAACCTCCTA	364	94°C 30 seconds	54°C 40 seconds	72°C 40 seconds