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. 2022 Apr 4;12(2):221–230. doi: 10.5455/OVJ.2022.v12.i2.10

Table 1. Changes in gene expression in E. coli F31 cultured in HD11 cells measured by microarray which were up-regulated by greater than 10-fold after 8 hours culture in HD11 cells.

Gene class Gene Function or product Mean change in expression 8 hours
Electron transport nadA Quinolinate synthetase A 13.65
norV Flavorubredoxin oxidoreductase 35.2
sdhA Succinate dehydrogenase flavoprotein subunit 11.17
ycdW Putative dehydrogenase 21.26
ydiR Predicted electron transfer flavoprotein, FAD-binding 12.13
ydjG Putative oxidoreductase 13.57
yehL Putative ATPase 14.96
ygcU Putative FAD containing dehydrogenase 11.41
- Putative dihydrolipoamide dehydrogenase 10.68
Carbon sources astA Arginine N-succinyltransferase 11.69
atoA Acetyl-CoA: acetoacetyl-CoA transferase, beta subunit 10.79
bglH Carbohydrate-specific outer membrane porin, cryptic 19
cdaR Carbohydrate diacid regulator 14.79
dadX Alanine racemase 2, catabolic 15.39
dcuB Anaerobic C4-dicarboxylate transporter DcuB 19.76
dgoD 2-oxo-3-deoxygalactonate 6-phosphate aldolase/galactonate dehydratase 13.05
eutA Ethanolamine utilization protein EutA 15.23
gclR Putative regulatory protein GclR 13.01
glcC Glc operon transcriptional activator 14
gltA Citrate synthase 13.18
gmd GDP-mannose 4,6-dehydratase 13.11
idnK Thermosensitive gluconokinase 10.84
metF 5,10-methylenetetrahydrofolate reductase 11.66
rbsA Putative ribose transport ATP-binding protein RbsA 13.98
- Putative hexuronate transporter 15.61
wzb Tyrosine phosphatase 13.49
xapR Santhosine operon regulatory protein 12.77
ybbA Putative ABC-type transport protein YbbA 14.75
ydjH Putative sugar kinase 10.84
ygfT Putative NADPH-dependent glutamate synthase beta chain 18.55
ygjK Putative glycosyl hydrolase 13.39
yieL Putative xylanase 13.72
yphF ABC-type sugar transport system, periplasmic component YphF 15.39
- Putative regulatory protein, GntR family 20.87
- Putative lactate dehydrogenase 10.93
Ion and other transport cniT Putative carnitine transporter CniT 11.34
cysA Sulfate transport ATP-binding protein CysA 11.56
cysW Sulfate/thiosulfate transporter subunit CysW 10.66
entS EntS/YbdA MFS transporter 10.33
fepC Ferric enterobactin transport ATP-binding protein FepC 13.5
fes Enterochelin esterase 12.13
livJ Leu/Ile/Val-binding protein precursor 12.22
mglC Galactoside transport system permease protein MglC 23.74
mntH Manganese transport protein MntH 18.8
nhaA Na+/H antiporter, pH dependent 10.86
sbp Periplasmic sulfate-binding protein 10.39
tauC Taurine transport system permease protein TauC 19.85
ybtU Yersiniabactin biosynthetic protein 17.66
ydjE Putative metabolite transport protein 13.9
yejB Predicted oligopeptide transporter subunit 10.29
Key regulatory functions bglJ BglJ 2-component transcriptional regulator 17.08
rseA Sigma-E factor, negative regulatory protein 12.6
ycdT Predicted diguanylate cyclase 22.83
ygaA Putative sigma-54-dependent transcriptional regulator YgaA 23.69
Surface and virulence functions bfr Bacterioferritin, iron storage and detoxification protein Bfr 17.69
csgE Assembly /transport component in curli production 11.24
eaeH Attaching and effacing protein, pathogenesis factor EaeH 12.16
fimD Outer membrane usher protein FimD precursor 13.79
sinH Putative intimin/invasin (SinH-like protein) 17.4
yfcV Putative Yfc fimbriae subunit YfcV precursor 19.6
- FimF protein precursor 11.67
wcaF Putative colanic acid biosynthesis acetyltransferase 11.11
yohK Putative seritonin transporter 10.25
betB Betaine aldehyde dehydrogenase, NAD-dependent 10.58
Stress responses betI Regulatory protein BetI 13.57
cpxP Periplasmic repressor of cpx regulon by interaction with CpxA 28.27
htrA Periplasmic serine protease Do, heat shock protein HtrA 19.53
osmC Osmotically inducible protein OsmC 13.14
proP Proline/betaine transporter 15.89
pspA Phage shock protein A 28.09
pspE Phage shock protein E precursor 20.53
Stress responses cpxP Periplasmic repressor of cpx regulon by interaction with CpxA 28.27
htrA Periplasmic serine protease Do, heat shock protein HtrA 19.53
osmC Osmotically inducible protein OsmC 13.14
proP Proline/betaine transporter 15.89
pspA Phage shock protein A 28.09
pspE Phage shock protein E precursor 20.53
recA DNA strand exchange and recombination protein with protease and nuclease activity 11.57
recN DNA recombination and repair protein RecN 14.4
recX Regulatory protein for RecA 12.03
rseA Sigma-E factor, negative regulatory protein 12.6
rspA Starvation sensing protein RspA 10.89
umuC Mutagenesis and repair protein UmuC 33.2
umuD Mutagenesis and repair protein UmuD 17.42
- Putative phage lysin 54.63
ygaA Putative sigma-54-dependent transcriptional regulator YgaA 23.68
Miscellaneous marA Multiple antibiotic resistance protein MarA 12.75
psiA PsiA—plasmid SOS inhibition 10.72
traN TraN—mating pair stabilisation protein 12.8
traR TraR—quorum sensing and plasmids 14.39
traW TraW—transfer pilus assembly F plasmid 15.02