Table 3. The effect of vaccination with bacterins produced by culture in NB or SMM-1 on the numbers of E. coli F135 in the tissues of chickens challenged intravenously. Log10 (± SD) of E. coli F135 in the blood, liver or spleen together with the number of chickens in each group from which the challenge strain was isolated.
| Day | Medium | Synthetic | NB | Control | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Blood | Spleen | Liver | Blood | Spleen | Liver | Blood | Spleen | Liver | ||
| Day 1 | Mean | 0.93 ± 1.92 | 0.84 ± 1.70 | 3.29 ± 0.31 | 0.94 ± 1.93 | 1.49 ± 1.08 | 2.97 ± 0.99 | 1.27 ± 1.66 | 1.93 ± 1.10 | 3.53 ± 0.55 |
| No. +ves | 1 of 5 | 1 of 5 | 5 of 5 | 1 of 5 | 3 of 5 | 5 of 5 | 2 of 5 | 4 of 5 | 5 of 5 | |
| Day 2 | Mean | <1±0.00 | <1 ± 0.00 | 1.9 ± 0.69 | 1.08 ± 2.23 | 1.16 ± 1.84 | 2.15 ± 1.70 | 1.01 ± 1.88 | 0.9 ± 1.16 | 2.56 ± 1.04 |
| No. +ves | 0 of 5 | 0 of 5 | 5 of 5 | 1 of 5 | 2 of 5 | 4 of 5 | 2 of 5 | 2 of 5 | 5 of 5 | |
| Day 4 | Mean | <1 ± 0.00 | <1 ± 0.00 | <1 ± 0.00 | <1 ± 0.00 | <1 ± 0.00 | 0.58 ± 0.72 | 0.88 ± 1.79 | 0.69 ± 1.36 | 0.78 ± 1.56 |
| No. +ves | 0 of 5 | 0 of 5 | 0 of 5 | 0 of 5 | 0 of 5 | 2 of 5 | 1 of 5 | 1 of 5 | 1 of 5 | |
| Day 7 | Mean | <1 ± 0.00 | <1 ± 0.00 | <1 ± 0.00 | <1 ± 0.00 | 0.32 ± 0.70 | 0.27 ± 0.54 | 0.22 ± 0.40 | 1.08 ± 1.50 | 0.37 ± 0.85 |
| No. +ves | 0 of 10 | 0 of 10 | 0 of 10 | 0 of 10 | 1 of 10 | 1 of 10 | 1 of 10 | 4 of 10 | 1 of 10 | |
| Total +ve/organ | 1 0f 25 | 1 of 25 | 10 of 25 | 2 of 25 | 6 of 25 | 10 of 25 | 6 of 25 | 11 of 25 | 12 of 25 | |
| TOTAL +ve | 12 of 75 | 18 of 75 | 29 of 75 | |||||||