Table 3. Thermal cycles’ conditions for cDNA amplification. For both genes, the nucleic acids were first denatured at 95°C for 5 minutes. After that, 35 thermal cycles followed. Lastly, the final extension at 72°C was for 10 minutes.

NLRP3			18S rRNA
95ºC	5 minutes		95ºC	5 minutes
94ºC	30 seconds	35 cycles	94ºC	1 minute	35 cycles
50ºC	30 seconds		50ºC	1 minute
72ºC	1 minute		72ºC	1 minute
72ºC	10 minutes		72ºC	10 minutes