Fig 3. Individually, oxidases cbb₃, bd-I, and bd-III support aerobic growth in V. cholerae.

(a-b) Combinatorial terminal oxidase deletion mutant growth in LB. Inoculums were prepared anaerobically and subsequently grown in aerobic and anaerobic conditions, respectively. Triple deletion mutant strains have a ‘+’ with an oxidase name (e.g. +cbb₃), indicating the sole remaining oxidase, with the other three oxidases disrupted by mutation. (c-d) Combinatorial terminal oxidase deletion mutant growth in LB. Inoculums were prepared aerobically and subsequently grown in aerobic and anaerobic conditions, respectively. (e) In vitro aerobic and anaerobic competition assay between Aero7 and wild type V. cholerae with competitive index scores calculated as [(Aero7_Output/WT_Output) / (Aero7_Input/WT_Input)]. Growth curves are an average of three biological replicates where error bars represent the standard error of the mean. Bars for in vitro competitions and expression data represent the arithmetic mean where error bars represent the standard error of the mean.