Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | CBA/Crl | Charles River | Strain code: 609 | Leicester breeding colony |
| Genetic reagent (M. musculus) | Kv3.1 knockout mouse on CBA back-ground (Kv3.1KO) |
Ho et al, PNAS 94, 1533–1,538. 1997. | CBA/CaCrl.Kcnc1tm1Joho/UoL | Strain originally created on 129/SV background and backcrossed onto CBA for >10 generations at the Preclinical Research Facility – University of Leicester |
| Genetic reagent (M. musculus) | Kv3.3 knockout mouse on CBA back-ground (Kv3.3KO) |
Espinosa et al., J Neurosci 21, 6657–6,665. 2001 | CBA/CaCrl. Kcnc3tm1Echa/UoL | Strain was originally created on C57BL/6 background and backcrossed onto CBA for >10 generations at the Preclinical Research Facility – University of Leicester |
| Commercial assay or kit | Illumina NextSeq500 High Output | Illumina | FC-404–2002 | Used for Cochlear Nucleus |
| Chemical compound, drug | Strychnine hydrochloride | Sigma Aldrich (MERCK) | S8753-25G | (0.5 µM) |
| Chemical compound, drug | Tetraethylammonium | Sigma Aldrich (MERCK) | 86614–25 G | (1 mM) |
| Chemical compound, drug | Fentanyl | Janssen | 6,001 | (0.05 mg/kg) |
| Chemical compound, drug | Midazolam | B Braun | 17206034 | (5.0 mg/kg) |
| Chemical compound, drug | Medetomidine | Vetoquil GmbH | Domitor | (0.5 mg/kg) |
| Antibody | Anti-Kv3.1b (rabbit polyclonal) |
Alomone | APC-014 | (1:1000) |
| Antibody | Anti-Kv3.3 (mouse monoclonal) |
Neuromab | 75–354 | (1:3000) |
| Antibody | Alexafluor488 Goat anti-rabbit (Goat polyclonal) |
Thermofisher | A-11008 | (1:1000) |
| Antibody | Alexafluor546 Goat anti-mouse (Goat polyclonal) |
Thermofisher | A-11003 | (1:1000) |
| Antibody | Anti-Kv3.1b (rabbit polyclonal) | Synaptic systems | 242 003 (Lot# 1–2) |
(1:300) |
| Antibody | Anti-Kv3.3 (rabbit polyclonal) | Alomone | APC-102 (Lot# APC 102AN0502) |
(1:300) |
| Antibody | Anti-Bassoon (mouse monoclonal) | Synaptic systems | 141 111 (Lot# 1–2) |
(1:300) |
| Antibody | Alexafluor488 Goat anti-mouse (goat polyclonal) | Invitrogen | A11001 (Lot# 21 40660) |
(1:300) |
| Antibody | Alexafluor546 Goat anti-rabbit (goat polyclonal) | Invitrogen | A11010 (Lot# 21 89179) |
(1:300) |
| Software, algorithm | Graphpad Prism 9.0.2 | Graphpad | RRID:SCR _002798 | |
| Software, algorithm | pClamp 10 software suite | Molecular Devices | RRID:SCR _011323 | |
| Software, algorithm | Zen Blue, 3.1 | Zeiss | RRID:SCR _013672 | |
| Software, algorithm | ABR Averager (Custom) |
Wellcome Trust Sanger Institute | ||
| Software, algorithm | AudioSpike | HoerrTech | https://audiospike.hz-ol.de/ | |
| Software, algorithm | MATLAB R2010a | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | Fiji | NIH ImageJ | RRID:SCR_002285 | |
| Other | Custom concentric bipolar stimulating electrodes | FHC | CBASD75 | |
| Other | Borosilicate glass capillaries | WPI | GC150F-7.5 |