FIGURE 4.

KLHDC7B-DT can bind with ILF2 protein and activate JNK/STAT3 signalling. (A) Silver staining of interacting proteins after the KLDHC7B-DT pulldown assay. (B) Mass spectrometry of ILF2. (C) Western blotting was used to detect ILF2 in the RNA pulldown product. (D) RIP experiments were performed using an antibody against ILF2 on lysates from HaCaT and Ker-CT cells; IgG was used as the control group. (E) Agarose electrophoresis detected KLDHC7B-DT in products from RIP. (F,G) mRNA and protein levels of ILF2 in KLDHC7B-DT knockdown or overexpression keratinocytes. (H) The efficiency of ILF2 knockdown was detected by Western blotting. (I) The mRNA levels of KLDHC7B-DT after ILF2 knockdown. (J) Activation of the STAT/JNK pathway after KLDHC7B-DT knockdown or overexpression. Data are shown as the means ± SD, ***p < 0.001. All experiments were repeated at least three times.