(A) Hep3B and Bel-7402 cells were treated with carfilzomib (0.1 μM) and sorafenib (7.5 μM) individually or in combination for 24 h and analyzed for cell death by flow cytometry. The experiments were performed in triplicate and data represent mean ± SD. *p < 0.05, ** p < 0.01, ***p < 0.001.
(B) Cleaved caspase-3, cleaved caspase-7, cleaved caspase-9 and cleaved PARP were used to analyze apoptotic cell death as detected by immunoblot. GAPDH was used as a loading control. Number indicates relative abundance (arbitrary unit).