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. Author manuscript; available in PMC: 2022 May 16.
Published in final edited form as: Mol Cancer Ther. 2018 Sep 17;17(12):2610–2621. doi: 10.1158/1535-7163.MCT-17-0541

Figure 3. Carfilzomib and sorafenib induce apoptosis of HCC cells in a caspase-dependent manner.

Figure 3.

(A) Hep3B and Bel-7402 cells were treated with carfilzomib (0.1 μM) and sorafenib (7.5 μM) individually or in combination for 24 h and analyzed for cell death by flow cytometry. The experiments were performed in triplicate and data represent mean ± SD. *p < 0.05, ** p < 0.01, ***p < 0.001.

(B) Cleaved caspase-3, cleaved caspase-7, cleaved caspase-9 and cleaved PARP were used to analyze apoptotic cell death as detected by immunoblot. GAPDH was used as a loading control. Number indicates relative abundance (arbitrary unit).