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. 2022 May 16;13:2688. doi: 10.1038/s41467-022-30436-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cytotoxicity studies of B16F10 cells cultivating with PCP-Mn-DTA, 1-MT, PCP-Mn-DTA@1-MT, and PCP-Mn-DTA@GOx@1-MT with or without glucose for 24 h and 48 h, respectively. b Tumor penetration and (c) endocytosis images of PCP-Mn-DTA@GOx@1-MT labelled with FITC in B16F10 MCSs after respective incubation at pH 6.8 or 7.4 for 4 h and 12 h, with images representative of 3 experiments. Nuclei and cytoskeleton were respectively stained with DAPI (blue) and ActinRed™555 (red). d Quantitative FCM analysis based on (c). Data represent mean ± SD (n = 6 biologically independent samples). P-values were determined by unpaired Student’s t-test (two-tailed), **p < 0.01. Scale bars: 100 µm for b, 50 µm for c.