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. 2022 May 16;13:2688. doi: 10.1038/s41467-022-30436-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a B16F10 cells incubated with FITC-labelled PCP-Mn-DTA@GOx@1-MT for different times, followed by staining with Lysotracker red to image the lysosome escape, with images representative of 3 experiments. b, c FCM and quantitative apoptosis analysis of B16F10 cells after difference treatments for 24 h. d–f Apoptosis-associated proteins in B16F10 cells after the treatment with indicated systems examined by western blot (d), and the related quantitative analysis of Bax/Bcl-2 (e) and Cytochrome C (f). g–i Microscopy images (g) and quantitative analysis of the wound healing and migration (h) as well as invasion assays (i) of B16F10 cells after the treatment with these systems. Data represent mean ± SD (n = 4 biologically independent samples). P-values were determined by unpaired Student’s t-test (two-tailed), **p < 0.01. Scale bars: 20 μm for a, 100 μm for g.