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. 2022 May 16;13:2417. doi: 10.1038/s41467-022-30185-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Reporter construct design of transient luciferase expression for the nucleus and mitochondria. b Transfection efficiency of the SWNT-PM-Peptide and pDNA was evaluated 18 h after infiltration using a Renilla luciferase reporter construct under the control of mitochondria-specific (Cox2) and nuclear (35S) promoters using whole seedling lysate soluble fractions. Data from 6 biologically independent samples (n = 6) are shown as the mean ± standard deviation. Statistical significance between individual samples was determined by one-way ANOVA with Brown-Forsythe and Welch test for multiple comparisons. P-values for the 35S promoter are 0.0087 between SWNT-PM and SWNT-PM-Cytcox, 0.0489 for SWNT-PM-Cytcox and SWNT-PM-CytKH9. For the Cox2 promoter, P-values are <0.0001 between SWNT-PM-SWNT-PM-Cytcox, and 0.0086 for SWNT-PM-Cytcox and SWNT-PM-CytKH9. For comparisons between 35S and Cox2 promoters, the P-values are 0.0265 for SWNT-PM-Cytcox, and 0.0033 for SWNT-PM-CytKH9. ns – not statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Evans blue assays for intact A. thaliana seedlings 18 h post infiltration with each respective NC, normalized to the absorbance of a boiled sample. A. thaliana infiltrated with DNA alone was used as a control. Data points from six biological replicates (n = 6) are represented as the mean ± standard deviation. Statistical significance between individual samples was determined by one-way ANOVA with Kruskal-Wallis test for multiple comparisons. ns – not statistically significant. Source data are provided as a Source Data file.