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. 2022 Apr 22;100(5):781–795. doi: 10.1007/s00109-022-02184-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Phosphorylation of nephrin serine 1146 in murine podocytes and nephrin serine 1146 and threonine 1120/1125 in diabetic mice (molecular mass markers are indicated in kilodalton (kDa)). a Western blot analysis of phosphorylation of nephrin serine 1146 in immortalized murine podocytes under low (5.5 mM) and high glucose (30 mM) conditions at given time points (n = 4 each). The results were quantified by densitometry and graphed as phospho-nephrin (p-nephrin) to total nephrin (nephrin) ratio–normoglycemic (5.5 mM) group was normalized to 100%. A total of 5.5 mM glucose vs. 30 mM glucose 4 h: 100.0 ± 8.4 vs. 216.4 ± 26.4% (*p < 0.05). b Western blot analysis of phosphorylation of nephrin serine 1146 and threonine 1120/1125 in nondiabetic (control) and diabetic (STZ) mice (day 5)–control was normalized to 100%: S1146 control (n = 3) vs. STZ (n = 4): 100.0 ± 6.0 vs. 162.2 ± 13.8% (*p < 0.05); T1120/1125 control (n = 3) vs. STZ (n = 4): 100.0 ± 2.9 vs. 191.9 ± 3.2% (****p < 0.0001)