Table 2.

Properties of the reported NAGase from various Trichoderma spp.

Trichoderma spp.	Name of enzyme; form; expression level	Mw (kDa)	Optimal pH; optimal temperature	pH tolerance; temperature tolerance (% activity remained)	Specify activityb (U mg−1 min−1); purification folds	Kmb	Refs.
T. virens strain mango	TvmNAG2; native; 5.4 U/ml	~ 68	pH 5 (pH 5–6, > 90% activity); 60–65 °C	pH 4–9 at 25 °C for 60 min (≧80%); 60 °C at pH 7 for 120 min (~ 85%)	10,698.3; 38.8	0.45 mM	In this study
T. reesei RutC30ΔU3	Nag1; Recombinant; 499.85 U/ml	~ 80	pH 4 (pH 3.5–6, > 60% activity); 60 °C	pH 4–6 for 60 min (≧80%); 60 °C for 120 min (~ 80%), 60 °C for 8 h (~ 60%)	319.89	69.41 ± 4 M	(Chen et al. 2015)
T. harzianum 1051/ NAGase	NAGase	36	pH 4; 37 °C	nd; 50 °C at pH 4 for 60 min ~ 50%	nd	8.06 mM	(De Marco et al. 2004)
T. harzianum Strain 39.1	NAGase; native	64, 118a	pH 5.6; 50 °C	nd	nd	1.27 µmol	(Ulhoa et al. 2001)
T. harzianum T198	Exochitinase; native	27.5 ~ 28	pH 3.5; 50 °C	pH 2–8 at 37 °C for 60 min (≧50%); 60 °C at pH 5.5 for 60 min (33%)	nd	850 µM c	(Deane et al. 1998)
T. harzianum P1	Nag1; native	72	nd	nd	11.8bd; 9	nd	(Lorito et al. 1994)
T. harzianum AF6-T8	NAGase; native; 3.1 U/ml	~ 69, 150a	pH 4–5.5; 50 °C	pH 3–6 at 37 °C for 60 min (≧80%); 65 °C at pH 5.2 for 30 min (~ 50%)	102; 13.2	0.24 mM	(Koga et al. 1991)

nd no data

^aEstablished by gel filtration

^bpNP-NAG as substrate

^cChitotriose as substrate

^dSpecific activity = nkatal mg⁻¹; one nkatal corresponds to the release of 1 nmol of p-nitrophenol per second