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. 2022 May 4;29(1):1–10. doi: 10.3727/096504021X16401852341873

Figure 6.

Figure 6

MYCN directly binds to the promoter regions of NeuroD1. (A) Schematic describing the MYCN responsive E-box (CACGTG) motif within the NeuroD1 gene promoter. TSS represents the transcription start site. (B, C) Chromatin immunoprecipitation (ChIP) assays were performed by using anti-MYCN antibodies in IMR-32 and CHP-134 cells, and an IgG antibody was used as the control. PCR was then performed to examine the bound DNA fragments. The results represent the mean ± SD (n = 3). **p < 0.001 versus the IgG group. (D) Human embryonic kidney (HEK) 293T cells were cotransfected with pRL-CMV (Renilla) control reporter vector, pGL4.74-NeuroD1 or pGL4.74-mut construct, MYCN expression vector, or control-expressing vector. The luciferase activity was measured and normalized by Renilla luminescence signals. The results represent the mean ± SD (n = 3). **p < 0.001 versus the control-expressing vector plus pGL4.74-NeuroD1-expressing group; #p < 0.01 versus the CMV-MYCN plus pGL4.74-NeuroD1-expressing group.