FIG. 2.

Inhibition of ACC synthase (bLE-ACS2) (A) and β-cystathionase from E. coli K-12 (B) by rhizobitoxine (RT), dihydrorhizobitoxine (DRT), and AVG. Enzymatic activities are expressed as percentages of activities in the absence of inhibitors, which were 6.28 nmol of ACC produced/h for the ACC synthase assay (A) and 50 nmol of pyruvate produced/h for the β-cystathionase assay (B). Each striped box indicates the range of rhizobitoxine concentrations that can be determined by the respective assay system. Concentrations of rhizobitoxine, AVG, and dihydrorhizobitoxine are shown in sample solutions (100 μl for the ACC synthase assay and 50 μl for the β-cystathionase assay).