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. 2022 May 3;10:850708. doi: 10.3389/fcell.2022.850708

FIGURE 3.

FIGURE 3

CircFGGY inhibits HCC growth, migration, invasion and EMT both in vitro and vivo. A-D. CCk8 assays (A), colony formation assays (B), scratch wound healing assay (C) and transwell invasion assay (D) assessed the cell proliferation, colony formation, migration and invasion potential in MHCC97H cells transfected with the circFGGY overexpression vector and HepG2 cells transfected with the circFGGY ASO (n = 3). Scale bar, 500 µm (C), 100 µm (D). (E) Western blot detected the expression of the EMT biomarkers in MHCC97H cells transfected with circFGGY overexpression vector and HepG2 cells transfected with ASO-circFGGY. (F) Nude mice inoculated subcutaneously with the circFGGY-overexpressing MHCC97H cells or NC cells. (G) Representative images of the MHCC97H xenograft tumors after 4 weeks of tumor growth in the circFGGY overexpression and NC groups. (H,I) The volume and weight of subcutaneous xenograft tumors (n = 5). (J) IHC analysis on Ki-67 expression in xenograft tumor tissues from the circFGGY overexpression and NC groups. Scale bar, 50 µm. Data were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 in two-way ANOVA (A,H) or independent Student’s t test (B,C,D,I).