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. 2022 May 3;10:850708. doi: 10.3389/fcell.2022.850708

FIGURE 5.

FIGURE 5

miR-545-3p promotes cell growth, migration, invasion, EMT and downregulates the expression of Smad7 in vitro. (A) qRT-PCR validated miR-545-3p upregulation in HepG2 cells transfected with miR-545-3p mimics and downregulation in HepG2 cells transfected with miR-545-3p inhibitors. B-D. CCk8 assays (B), scratch wound healing assay (C) and transwell invasion assay (D) assessed the cell proliferation, migration and invasion potential in HepG2 cells transfected with miR-545-3p mimics or inhibitors. Scale bar, 500 µm (C), 100 µm (D). (E) qRT-PCR detected the RNA expression of Smad7 in HepG2 cells transfected with miR-545-3p mimics or inhibitors. (F) Western blot detected the expression of Smad7 and E-cadherin in HepG2 cells transfected with miR-545-3p mimics or inhibitors. Data were shown as mean ± SD, (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 in independent Student’s t test (A,C–F) and in two-way ANOVA (B).