Table 1.
Detection | Test kits | Target | Method | Amplification | Sensitivity a) | Time to result [min] b) | Manufacturer | Website |
---|---|---|---|---|---|---|---|---|
HIV‐1/HIV‐2 p24 | RocheElecsys HIV Combi PT | p24 antigen | Sandwich ELISA | N/A | ≤2 IU mL−1 | 27 | Roche | www.roche.com |
Dengue (serotype 1–4) | Abcam human anti‐dengue virus IgG ELISA | IgG | Indirect immunoassay | N/A | >90% | 90 | Abcam | www.abcam.cn |
HBV | Bayer ADVIA Centaur HCV assay | HBsAg | Chemiluminescent immunoassay | N/A | 0.12 ng mL−1 | 29 | Bayer | www.bayer.com |
Respiratory syncytial virus (RSV) | Anti‐Respiratory Syncytial Virus (RSV) IgM ELISA kit | RSV IgM | Enzyme immunoassay | N/A | N/A | 90 | Antibodies | www.antibodies‐online.cn |
Avian influenza | ID Screen influenza competitive subtype‐specific kits | N/A | Competitive ELISA | N/A | 93% | 150 | ID | www.id‐vet.com |
ZIKV | TaqPath Zika Virus kit | N/A | Nucleic acid amplification | RT‐PCR | 1 copies mL−1 | <1 h | Thermofisher | www.thermofisher.com |
MERS‐CoV | RealStar MERS‐CoV Kit | N‐gene | Nucleic acid amplification | RT‐PCR | 2.8 × 10−3 PFU | <1 h | Altona | www.altona‐diagnostics.com |
2019‐nCoV, SARS‐CoV | QIAGEN One‐Step RT‐PCR Kit (2019‐nCoV, SARS‐CoV) | E gene | Nucleic acid amplification | RT‐PCR | 5.2 copies mL−1 | <30 min | QIAGEN | www.Qiagen.com |
N gene | 8.3 copies mL−1 | |||||||
Influenza virus | ABI 7500 influenza A‐kit | M gene | Nucleic acid amplification | RT‐PCR | 10–100 copies mL−1 | <30 min | Thermofisher | www.thermofisher.com |
RSV | BioRad virus RT‐PCR Kit | F gene of RSV | Nucleic acid amplification | RT‐PCR | 1.4 × 10–1.4 × 106 pg mL−1 | 3.5–4 h | Bio‐Rad | www.bio‐rad.com |
HAdV HIV‐1 SARS‐CoV‐2 HSV1/HSV2 |
Altona RealStar Adenovirus PCR Kit 1.0 NucliSens HIV‐1 EasyQ 2.0 SARS‐CoV‐2 visual RT‐LAMP kit BD Viper LT |
HAdV (hexon) N/A N gene N/A |
Nucleic acid amplification Nucleic acid amplification Dye method Fluorescent energy transfer |
One‐Step RT‐PCR NASBA LAMP SDA |
<1.7 log10 copies mL−1 10 copies mL‐1 10 copies mL−1 96.8% |
2 h <3 h 40 min <4.5 h |
Altona Biomeriex Biorab BD |
International unit: IU (WHO International Standard Unit); plaque‐forming units: PFU; tissue culture infective dose: TCID;
time to result depends upon the particular assay.