Table 2.
Examples of commercial POCT platforms that are available to market for virus detection
Detection | Production | Target | Method | Amplification | Sensitivity a) | Time to result [min] d) | Manufacturer | Website |
---|---|---|---|---|---|---|---|---|
HIV‐1 | m‐PIMA HIV‐1/2 VL | HIV‐1 group M, group N and group O, HIV‐2 | Microfluidic chips | Real‐time qRT‐PCR | 800 copies mL−1 | <70 min | Abbott | www.abbott.com |
HIV‐1 | Xpert HIV‐1 VL | HIV‐1 group M, group N and group O | Microfluidic chips | Real‐time qRT‐PCR | 40–107 copies mL−1 | <90 min | Cepheid | www.cepheid.com |
HIV‐1/HIV‐2/HCV/HBV | Cobas TaqScreen MPX Test | N/A | Nucleic acid amplification | Multiple real‐time PCR | 50 copies mL−1 (HIV), b) 50 copies mL−1 (HCV), 10 copies mL−1 (HBV) c) | <60 min | Roche | www.roche.com |
HIV‐1/HCV/HBV | Ultrio | N/A | Nucleic acid amplification | Multiple real‐time PCR | 44.6 IU mL−1 (HIV), 10.44 IU mL−1 (HBV),3.01 IU mL−1 (HCV) | <60 min | Novartis | www.novartis.com |
HIV‐1/HIV‐2 | Alere q HIV‐1/2 | HIV‐1 group M, group N and group O, HIV‐2 | Nucleic acid amplification | Real‐time PCR | 2937 IU mL−1 | <60 min | Abbott | www.abbott.com |
Influenza A/B | Cobas Liat Influenza A/B | N/A | Microfluidic chips | Real‐time PCR | 10−2–10−1 TCID50 mL−1 (Influenza A)/10−3–10−1 TCID50 mL−1 (Influenza B) | 15–20 min | Roche | www.diagnostics.roche.com |
Influenza A/B | ID NOW INFLUENZA A & B 2 | N/A | Microfluidic chips | Isothermal (RPA) | N/A | <13 min | Abbott | www.abbott.com |
Influenza A/B | BD veritor system Flu A+B | N/A | Immunochromatographic strip | N/A | N/A | 15–20 min | BD | www.bd.com |
Ebola virus | Film array NCDS BT‐E | N/A | FilmArray NGDS BTE assay pouches | Real‐time qRT‐PCR | 100 PFU mL−1 | 1 h | BioFire | www.biofireinc.com |
Ebola virus | Lightcycler,COBAS Z480 | N/A | High pure viral nuclenic acid Kit | Real‐time qRT‐PCR | 4781 PFU mL−1 | 3 h | Roche | www.roche.com |
Ebola virus | LA‐200 | Nucleoprotein coding genes | Nucleic acid amplification | Isothermal (LAMP) | 104–105 copies mL−1 | <60 min | Eiken | www.eiken.co.jp |
HIV | LIAISON 200 tests 310260 XL murex HIV Ab/Ag | p24 antigen | Chemiluminescence immunoassay | N/A | 27.4 pg mL−1 | <60 min | Diasorin | www.diasorin.com |
HIV‐1/HIV‐2 | HIV(I+2) rapid test strip | N/A | Rapid test strip | N/A | N/A | <15 min | Kehua Bio‐engineering | www.skhb.com |
Syphilis | Rapid test strip | Syphilis antigen | Rapid test strip | N/A | N/A | 15–20 min | Wondfo | www.wondfo.com.cn |
Ebola virus | ReEBOV antigen rapid test | Antigen VP40 | Rapid test strip | N/A | 1.0 × 106 PFU mL−1 | <15 min | Corgenix | www.corgenix.com |
Ebola virus | OraQuick Ebola rapid antigen test | Antigen | Rapid test strip | N/A | 1.64 × 106 PFU mL−1 | 30 min | OraSure | www.oraSure.com |
International unit: IU (WHO International Standard Unit); Plaque‐forming units: PFU; Tissue culture infective dose: TCID;
diagnostic sensitivity at limit of detection with WHO 3rd International Standard, 1000 copies mL−1;
1 IU mL‐−1 ∼ 5.6 copies mL−1, which is defined by the WHO;
time to result depends upon the particular assay.