Table 1.
Allergens identified by isoform-specific peptides in 52 apple samples (Malus × domestica Borkh). Each isoform is presented in a different line. Related protein groups, the percentage of amino acid identity for Mal d1 isoforms to Bet v 1a and detected specific peptide sequences are presented.
| Protein name/ NCBI accession number |
Protein group | Pairwise identity to Bet v1 (%) | Matched specific peptides (Sequence position) |
|---|---|---|---|
| CAD32318.1 | Mal d1.02 | 57 | GVYTYENEYTSEIPPPR (2–18) |
| AEF38456.1 | Mal d1.02 | 56 | LFMAFVLDADNLIPK (19–33) |
| AEF38460.1 | Mal d1.02 | 56 | NITFGEGSQYGYVK (56–69) |
| XP_008377426.1 | Mal d1.03 | 59 | HKIDGVDKDNFVYK (70–83) LVAYGSGSVIK (105–115) |
| AAX21000.1 | Mal d1.03 | 56 | LMASGSGCVIK (105–115) HRIDGVDKDNLVYK (70–83) IDGVDKDNLVYK (72–83) |
| AAX21003.1 | Mal d1.03 | 60 | LVAAGSGSVIK (105–115) |
| AAX21002.1 | Mal d1.03 | 60 | LVAASSGSVIK (105–115) |
| AAX21008.1 | Mal d1.03 | 59 | LVASDNGSIIK (105–115) STSHYYTKGDVEIKEEHVK (116–134) |
| CBL94173.1 | Mal d1.03 | 60 | LVASDSGSIIK (105–115) |
| AAX21007.1 | Mal d1.03 | 60 | LVASSNGSVIK (105–115) |
| XP_008377311.1 | Mal d1.03 | 57 | YSVIEGDAITETIEK (84–98) YSVIEGDAITETIEKISYETK (84–104) |
| AAX20991.1 | Mal d1.06 | 53 | LYNALVLDADNLILK (19–33) |
| XP_008351173.1 | Mal d1.06 | 56 | HKVDGIDKDNFVYK (70–83) VDGIDKDNFVYK (72–83) |
| XP_008390601.1 | Mal d1.06 | 56 | STSHYHTKEDVEIK (116–129) STSHYHTKEDVEIKEEHVK (116–134) EDVEIKEEHVK (124–134) |
| AAS00045.1 | Mal d1.06 | 55 | LYYALVLDADNLLPK (19–33) VGKDKAHDLFK (135–145) DKAHDLFK (138–145) |
| AAX18308.1 | Mal d1.07 | 60 | HRIDGVDKENFVYQYSVIEGDAISETIEK (70–98) |
| CBL94174.1 | Mal d1.07 | 60 | HRIDGVDKDNFVYQYSVIEGDAISETIEK (70–98) |
| AAX20992.1 | Mal d1.07 | 59 | HKIDGVDKDNFVYQYSVIEGDAISETIEK (70–98) |
| CBL94177.1 | Mal d1.08 | 58 | LFNATALDGDELIAK (19–33) SIEILEGDGGVGTVQK (41–58) IIFGEGSTNGYVK (57–69) RIDVIDKDNFVYK (71–83) IDVIDKDNFVYK (72–83) ISYETTLVASGSGSIIK (99–115) GDVEINEEHLK (124–134) |
| CBL94148.1 | Mal d1.11 | 33 | MFNALILDAHNICPK (19–33) IDALDKEALSCTYTFIESDATDHLLDKLEYITYDVK (72–107) EALSCTYTFIESDATDHLLDKLEYITYDVK (78–107) |
| XP_008346874.1 | Mal d1.11 | 34 | MFNALILDSHNLCPK (19–33) IDALDKEALSCSYTFIESDASDHLMDKLEYITYDVK (72–107) |
| ABB96482.1 | Mal d3 | – | NGGAVPPACCNGIR (43–56) TINSLAR (57–63) |
| AAT80665.1 | Mal d3 | – | SLAGSVSGVNPGNVESLPGK (77–96) |
| AAX19856.1 | Mal d4 | – | KTGQDLVFGIYEEPLTPGQCNMIVER (96–121) |
| XP_008337609.1 | Mal d4 | – | LGDYLVEQGL (122–131) |