Table 2.
Peptides overabundant in allergenic apple genotypes of all three harvest years. Overabundant peptides with a statistically significant p-value (<0.05), resulting from a T-Test analysis comparing the peptide abundance of each group III genotype to that of group I, are shown. Only positive fold changes indicating an increase in abundancy of the presented peptides for each allergenic genotype (group III) are shown. Specific peptides are shown in bold font.
| Harvest year | 2017 | 2018 | 2019 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotypes | p160 | p186 | p36 | p167 | p29 | p48 | p78 | GD | p17 | p36 | p186 | ||
| Mal d1 [µg/g FW] | - | - | - | - | - | - | - | - | 8.33 ± 1.10 | - | 0.67 ± 0.31 | ||
| Peptide sequence (position within the protein sequence) | Protein name/NCBI accession number | Protein group | |||||||||||
|
HKVDGIDKDNFVYK (70–83) VDGIDKDNFVYK (72–83) |
XP_008351173.1 | Mal d1.06 | 44.67 2.51 |
5.37 | 12.02 4.47 |
||||||||
| LVASSNGSVIK (105–115) | AAX21007.1 | Mal d1.03 | 4.17 | ||||||||||
|
LVASDNGSIIK (105–115) STSHYYTKGDVEIKEEHVK (116–134) |
AAX21008.1 | Mal d1.03 | 3.98 | 4.79 2.57 |
29.51 | 3.98 | |||||||
|
LYYALVLDADNLLPK (19–33) DKAHDLFK (138–145) |
AAS00045.1 | Mal d1.06 |
3.02 |
2.82 |
5.62 | ||||||||
| LVAASSGSVIK (105–115) | AAX21002.1 | Mal d1.03 | 58.88 | 15.49 | 18.20 | ||||||||
| LVAAGSGSVIK (105–115) | AAX21003.1 | Mal d1.03 | 4.27 | ||||||||||
| GDFEIKEK (124–131) |
AEF38445.1 AFC65129.1 |
Mal d1.06 Mal d1.06 |
3.31 | ||||||||||
| TVEILEGDGSVGTIK (41–55) TVEILEGDGSVGTIKK (41–56) |
AAS00045.1 AAX20977.1 |
Mal d1.06 Mal d1.03 |
4.17 12.59 |
5.50 8.91 |
3.72 5.31 |
3.63 4.37 |
3.09 |
||||||
| VTFGEGSQLGFVK (57–69) LVASPDGGSIVK (106–117) |
CBL94138.1 XP_008340764.1 |
Mal d1.10 Mal d1.10 |
2.57 |
2.88 |
3.55 |
||||||||
| SDVEIKEEHVK (124–134) |
AAX20997.1 CAA96534.1 |
Mal d1.03 Mal d1.03 |
2.95 | ||||||||||
| LIESYLKDHPDAYN (146–159) |
AAD26552.1 AAD26553.1 AAD26554.1 AAD29671.1 AAX20917.1 AEE38287.1 P43211.2 CAD32318.1 |
Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.02 |
16.98 | 11.22 | 11.48 | 2.82 | |||||||
| QAEILEGNGGPGTIK (41–55) QAEILEGNGGPGTIKK (41–56) |
AAD26552.1 AAD29671.1 AAD26554.1 AAD26553.1 AEE38287.1 AAD26546.1 AAD26555.1 AAX20917.1 CAA88833.1 P43211.2 |
Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 Mal d1.01 |
5.50 |
12.30 7.76 |
3.09 3.31 |
||||||||
| VCPAPLQVK (162–170) SACLAFGDSKYCCTPPNNTPETCPPTEYSEIFEK (181–214) YCCTPPNNTPETCPPTEYSEIFEK (191–214) ITFTNNCPNTVWPGTLTGDQKPQLSLTGFELASKASR (27–63) |
AAC36740.1 NP_001315714.1 |
Mal d2 | 11.30 | 3.31 29.51 6.92 |
2.63 2.51 |
||||||||
| SLAGSVSGVNPGNVESLPGK (77–96) | AAT80665.1 | Mal d3 | 5.75 | ||||||||||
| LGDYLVEQGL (122–131) | XP_008337609.1 | Mal d4 | 3.24 | ||||||||||
| KTGQALVFGIYEEPLTPGQCNMIVER (96–121) |
XP_008365251.1 AAD29412.1 |
Mal d4 Mal d4 |
4.90 | ||||||||||