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. 2022 Mar 13;2(6):2100124. doi: 10.1002/smsc.202100124

Figure 3.

Figure 3

Mass spectrometry analysis and kinetic studies of 3CLpro by Oridonin. a) The molecular ion profile of 3CLpro treated with DMSO. b) The molecular ion profile of 3CLpro treated with Oridonin. The theoretical molecular mass is 34 865 Da (average mass) for 3CLpro and 35 230 Da (average mass) for 3CLpro treated with Oridonin. The Δ mass is equal to the molecular mass of Oridonin. c) The 3CLpro was incubated with five different concentrations of Oridonin, respectively. For the protease activities of each concentration at different incubation time was measured by the FRET protease assay and plotted against different incubation time. d) The resulting k obs values were plotted versus Oridonin concentrations to calculate the k inact and K i values.