Table 1.
The eight positive hits of thermal shift assay and their inhibitory effect on 3CLpro
| Compound | Structure | ΔT m [°C]a) | Relative enzyme activity [%]b) |
|---|---|---|---|
| +(–)Catechin |
|
3.2 | 105 |
| Sennoside B |
|
3.0 | 97 |
| Ddabvi |
|
−5.1 | 108 |
| EGCG |
|
−6.3 | 44 |
| SAB |
|
−6.4 | 96 |
| SAA |
|
−8.3 | 36 |
| Esculetin |
|
−9.4 | 95 |
| Oridonin |
|
−11.1 | 28 |
a)
ΔT m is the shift between the ΔT m values of 3CLpro with and without compounds.
b)
Relative enzyme activity represents the ratio of the enzymatic activity of 1.2 μmol 3CLpro at 50 μmol compound. The data are the average of two independent experiments.